Hope Australia



Individuals with hope, receiving personalised treatment, and who are a member of a well run support group live twice as long as those without this attitude & support.

Hair Analysis from our affiliated European Laboratory: 

Contact us for instructions.

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Disclaimer: The information on this website is presented for educational purposes only. It is not intended as a substitute for the diagnosis, treatment, or advice of a qualified, licensed medical professional. The facts presented are offered as information only, not medical advice, and in no way should anyone infer that we are practicing medicine. Seek the advice of a medical professional for proper application of this material to any specific situation.

No statement on this website has been evaluated by the Food and Drug
Administration. Any product mentioned or described on this website is not intended to diagnose, treat, cure, or prevent any disease. If you purchase anything through a link in this email or website, you should assume that we have an affiliate relationship with the company providing the product or service that you purchase, and that we will be paid in some way. We recommend that you do your own independent research before purchasing anything.

35+ years of clinical & environmental analysis – worldwide

Hope Trace Minerals, Australia, was founded in 1975, specialising in the analysis of minerals and toxic metals.

Our affiliated European laboratory tests human, animal and environmental samples such as water and soil. They analyse the metal concentration in a variety of specialty samples such as cosmetics, medication, nutrients, food, even tissues.

Ask us if you have questions or request instructions to complete a hair analysis for you health professional. Your inquiry will be appreciated, Contact Us 

Our affiliated lab is actively pursuing research and have established reference ranges for urine challenge tests involving various chelating agents.

They search for, and provide reliable answers to environmental problems.

They are involved in Humanitarian projects.

They utilize state-of-the art equipment, and regularly engage in round robins with excellent results. 

Accreditations/Memberships of our affiliated European Laboratory who process our samples:

  • AKS-PL-20918
  • ISO/IEC 17025:2005
  • Recipe Reference Laboratory 2011
  • Member, Relana© Quality Circle (membership is provided to quality analytical laboratories that have met highest expert requirements)
  • QS-System for Foods
  • VDI-DIN-Certificate for Testing of Pharmaceuticals

Hope Trace Minerals: European Hair Analysis

35 years of clinical & environmental diagnostics

Send sample to:

P O Box 137 Parkville VIC 3052 Australia Phone 0412 994 001

hair@smile.org.au   www.smile.org.au

Hair submission form:


Requesting Clinic/Doctor


Patient Name


Street                                                                                                                   City                     


State              Country                                    Phone                              Fax                            


Email                                                                                        Date of Birth                          Sex M/F


Date                                  Patient Signature


Requested Profile

Standard Profile    35 Elements                                                               A$160

Tested Elements:

Aluminum, Antimony, Arsenic-total, Barium, Beryllium, Bismuth, Boron, Cadmium, Calcium, Chromium, Cobalt, Copper, Germanium, Iodine, Iron, Lead, Lithium, Magnesium, Manganese, Mercury, Molybdenum, Nickel, Palladium, Platinum, Selenium, Silver, Strontium, Thallium, Tin, Titanium, Tungsten, Uranium, Vanadium, Zinc, Zirconium

Extended Profile    55 Elements                                                              A$190

Tested elements are standard profile elements plus

Cerium, Cesium, Dysprosium, Erbium, Europium, Gadolinium, Gallium, Iridium, Lanthanum, Lutetium, Praseodymium, Rhenium, Rhodium, Ruthenium, Samarium, Tantalum, Tellurium, Thorium, Thulium, Ytterbium

Test Material

( ) Head hair untreated  ( ) Pubic hair  ( ) Beard sample   ( ) Nail Sample


Credit Card ( ) Visa ( ) Mastercard Card Number_______________

Valid thru (MM/YY) ___/___ 3 digits on back of card ______


PayPal    Payment to address noelc@smile.org.au  Date   /    /

 Pre-payment or credit card is needed, otherwise samples will be held until payment is received

 Sampling Instructions

Hair provides a record of past and current trace element levels. As hair grows, nutrient and toxic elements are deposited from the blood stream into the hair follicle and hair shaft. Once a trace element has been incorporated into the hair, it remains fixed. To measure these values reliably and with good reproducibility, the following criteria applies:

Untreated head hair that is cut from the occipital area (back of head) is the preferred sample.

Substitutes for head hair are pubic hair or clippings of finger nails (underarm hair is not acceptable).

Because some sample loss results from thorough washing, an adequate amount of sample (about 2-3 Teaspoons filled with hair) is needed for the analysis.

DO NOT MIX different sample types (i.e. hair with nails).

Hair roots are NOT needed. Hair that has been chemically treated (“permed”, dyed, bleached, or otherwise treated) WILL NOT provide accurate results. If the hair has been treated, it should be allowed to grow for at least 2-3 months before new sampling.

Hair analysis results are falsely elevated by:

Colouring or tinting agents, which often elevate a number of trace elements or toxic metals. Since today’s cosmetic industry uses many different chemical agents, we have no way of accurately determining their effects. Bleached and/or “Permed” hair results in altered levels of calcium, magnesium, zinc, copper, nickel and other metals.

 Procedure to prepare sample:


Part the hair in the middle of the back of the head, and pull it out and up.

Cut a small 1.5 to 2 inch (4.5 to 5.5 cm.) strand of hair close to the head. DISCARD ends of long strands and KEEP less than 2 inches (less than 5.5 cm) closest to the scalp.

Place hair in sampling envelope, fill out the Sampling Instructions Form with the appropriate information and send to Hope Australia P O Box 137 Parkville VIC 3052 Australia.

 Short hair

Trim a minimum of 0.300 grams of hair from the back of the head. Use thinning scissors, if possible.

Place hair in sampling  envelope (paper), fill out the sampling Instructions form with the appropriate information, and send to:

Hope Australia P O Box 137, Parkville, VIC 3052, Australia


Pubic hair

Trim a minimum of 0.300 grams of pubic hair with normal scissors.

Place hair in paper envelope, fill out the Sampling Instructions Form with the appropriate information and send to

Hope Australia P O Box 137 Parkville VIC 3052 Australia

Under construction


We need a minimum of 0.200 grams (200mg) of unpolished nails. This equals about 1/2 of a filled teaspoon.

Place sample in a paper or plastic envelope, fill out the Instructions Form and send to

Hope Australia P O Box 137 Parkville VIC 3052 Australia

Send report to ( ) Doctor ( ) Patient Both addresses A$10 surcharge for both addresses

Send report via (  ) E-Mail  (…) Fax  (…) Express Post A$ 10 surcharge for Express Post

Doctor details: Please Email hair@smile.org.au if you would like hair kits to be sent to your practice

Name, and Address of Professional


Professional Association Membership Number:


Professional’s  Phone                    Fax                      Email


We provide laboratory diagnostics for orthomolecular and environmental physicians interested in prevention and cause-related treatment. We are specialised in the analysis metals and related diagnostics. We test biological and environmental samples, serving the private, clinical and industrial sector.

Hope Trace Minerals was founded by Prof.  Campbell in 1975, serving medical and veterinary physicians,  naturopaths and other non-medical therapists.

The European interdisciplinary team is actively engaged in serving  international clientele. They are involved in environmental and medical studies, and serve as a reference centre for other laboratories.

Their accuracy, efficiency, reliability and cost-effectiveness has, and is appreciated by their international clientele.

Proficiency tests

Accredited laboratories need to participate in proficiency test, also called Round Robin tests to assure measurement accuracy.

A round robin test is an inter laboratory test (measurement or analysis) performed independently several times. It is a measure of a laboratory’s analytical performance.

In total, 72 elements were tested in urine, plasma and whole blood.

Their sensational results were a score of 100%!

This indicates the excellency of their work. They are proud of these results and thank all their co-workers for their commitment.




Laboratory News

Compact News

Reference and Orientation Ranges

More is not better

DMSA oral – Bioavailability and dose-dependent metal excretion

Guide to Metal Toxicology

Combination treatments

Medical Workshops and Conferences

Conferences and Workshops 2016

Studies and Analyses

EDTA and TACT (Trial to Assess Chelation Therapy), second trial








Laboratory News

Compact News

We realize how precious time is, and how little time we have to read lengthy articles. Therefore, we summarize important news. Contact us for more information.

Reference and Orientation Ranges

We have updated existing ranges and added new Orientation Ranges for combination treatments such as ZnDTPA and DMPS.

This will allow a more precise evaluation and comparison of test results.

More is not better

We have statistically evaluated the hypothesis that more of a chelating substance increases urinary excretion values accordingly. From our data, we can postulate that this is not the case. We verified the following data supplied by Heyl, Berlin (manufacturer of Dimaval):

DMPS oral – Urinary Copper excretion

DMPS oral in mg/kg

Copper excretion in %










The manufacturer of Dimaval lists a bioavailability of about 50% for oral DMPS. This is in agreement with our urine data comparison of DMPS iv vs. DMPS oral.

The statistical evaluation of urine excretion data obtained after the intravenous application of 1 ampule DMPS (250mg active substance) vs 5 ampules DMPS (1250mg active substance), administered one after another at the same sitting, showed little effect. In fact, the median suggests that the application of more than one ampule DMPS provides no advantage. With the exception of copper, selenium, zinc and urine creatinine level, the median concentration for toxic elements in urine stayed the same or decreased slightly.

DMSA oral – Bioavailability and dose-dependent metal excretion

The bioavailability of a chelating substance is affected by the route of administration and the pH of the environment in which it circulates. International citations list the bioavailability of DMSA between 20 and 50%. We assume that gastrointestinal function is an important factor in determining an oral chelator’s bioavailability, its metal binding and urinary excretion. Oral chelators first find and bind metals located in the GI Tract, therefore fecal metal binding influences urinary metal excretion. GI ‘cleansing’ may have to come before a urine provocation or challenge test. Attention to pH seems warranted.

We compared urinary metal concentration of a 500mg oral dose with that of 1000mg and found little difference in metal excretion. See Table below

DMSA oral

N = # of Tests




















Median value in mcg/g Creatinine

Guide to Metal Toxicology

Our new German “Handbuch der Metall Toxikologie“, is a cookbook version of a chelation therapy textbook. It provides updated protocols for various chelating agents, statistical data indicating the efficacy of metal binding for each chelator, data based suggestions for treatment schedules based on provocation test results and practical treatment tips.

The English version ‘The Handbook of Metal Toxicology’ is available soon.

Combination treatments

There is no advantage in combining chelating agents with similar function (i.e. vicinal dithiol such as DMSA and DMPS) especially if applied in the same manner (i.e. oral).

When EDTA iv is combined with oral DMSA, the vascular and the GI tract are detoxified at the same time; however statistics involving urinary metal tests do not provide evidence that the combination treatment increased renal metal excretion more than the single administration of intravenous EDTA would. Fecal metal testing can provide answers regarding fecal detox.








Medical Workshops and Conferences

International Conferences & Workshops 2016


Seminar Melbourne

Update on Chelation, Diagnostics & Treatment

Melbourne, Australia (English)


Seminar Brisbane

Update on Chelation, Diagnostics & Treatment

Brisbane, Australia (English)


Seminar Sydney

Update on Chelation, Diagnostics & Treatment

Sydney, Australia (English)


Nonmedical Seminar

Update on Chelation, Diagnostics & Treatment

Nuremberg, Germany (German)








Studies and Analyses

EDTA and TACT (Trial to Assess Chelation Therapy), second trial

The National Center for Complementary and Integrative Health (NCCIH) of the National Institutes of Health (NIH) has awarded $800,000 to Mount Sinai Medical Center of Florida and the Duke Clinical Research Institute to initiate a planning year for the second Trial to Assess Chelation Therapy (TACT2).

We suggest that the metal detoxification effect of EDTA is included in the study. EDTA chelation removes toxic metals from the vascular system, thereby reducing inflammation and increasing the blood flow – all of which can be documented.


Diagnostic Humans

Analytical Excellence

Growing environmental problems have increased the need for reliable environmental medicine at reasonable cost. Global demands require innovation in laboratory diagnostics, and in over 40-years of serving doctors worldwide we have achieved recognition for providing reliable analytical services.

We use the most modern analytical instrumentation and methods, and regularly participate in laboratory round robins. We have achieved excellent results, which are open for you to see.

Our qualified analytical staff utilizes the highest quality control standards, and continues to engage in method development and research.

In cooperation with IBCMT (International Board of Clinical Metal Toxicology) we regularly teach workshops on laboratory diagnostics as they apply to chelation therapy.

We are dedicated to serve you and your patients.

International Services

Toxins are our specialty, and the laboratory tests we provide relate to chronic intoxication and related orthomolecular issues, both for humans and animals.

We test metals in environmental samples such as water, we analyse medication, human tissue, nutrients, food and cosmetics.

We work internationally and receive samples from around the world. Sample submission from countries afar is no problems, simply because metals, if samples properly, do not spoil. For instance, a blood, urine, or water sample for metal testing is not time-sensitive.

Ask us if you have questions about sample submission, or send a mail to hair@smile.org.au

Test kits are provided upon request.

Why Biomonitoring?

Human Biomonitoring is a scientific technique for assessing human exposures to environmental agents and their effects, based on sampling and analysis of an individual’s tissues and fluids. While blood, urine, breast milk and expelled air are most commonly measured, hair, nails, fat, bone and other tissues may also be sampled. This technique takes advantage of the knowledge that environmental agents that have entered the human body leave markers reflecting this exposure. The marker may be the agent itself or a breakdown product, but it may also be some change in the body resulting from the interaction of the agent or its breakdown product(s) with the individual, such as alterations in the levels of certain enzymes or other proteins which may lead to modifications of normal body processes

Reference Ranges (RR)

In health-related fields, a reference range (RR) usually describes the variation of a measurement or value in healthy individuals. The standard definition of a RR in laboratory medicine is usually defined as the prediction interval between which 95% of values of a reference group fall into.

Detection Limit (DL)

The DL, also known as LOD (Limit of Detection), is the lowest amount of an analyte in a sample that can be detected, but not necessarily quantitated as an exact value. The DL may be expressed as

DL = 3.3*SD/S

where SD = standard deviation of the response and S = the slope of the calibration curve.

Limit of Determination

This is the lowest concentration or value of an analyte that can be identified and quantitatively measured.

Blood Metal Analysis

Blood is a transporting and circulation system, providing tissues with minerals, trace elements including toxins. Metals circulate in the blood stream for approximately 72 hours, after which they are naturally excreted or deposited in various body tissues.

Elevated blood metals indicate immediate exposure, reduced concentrations of essential minerals and trace elements reflect an inadequate intake and may be a sign of a nutritional deficiency.

Whole blood levels reflect the intra- and extracellular metal concentration; serum and plasma levels indicate extracellular levels only.

View Whole Blood Sample Report

To confirm acute metal toxicity, further diagnostics may be needed such as an appropriate provocation test.

To confirm or rule out longterm exposure, hair analysis is recommended.

Nutrient Metals

Nutritional deficiencies reduce detoxification potentials.

A zinc or copper deficiency reduces SOD (Superoxide Dismutase) function, thus blocking the Phase 1 Detoxification Cycle. Chelating Agents such as EDTA or DMPS easily bind trace elements such as zinc and copper.

Manganese deficiency reduces the function of the detoxification enzyme MnSOD; manganese intoxication can induce Pseudo Parkinson (which is easily treated via detoxification). Blood tests are important to adequately diagnose the nutritional status.



Whole Blood Metals

Needed Material: 3-5ml Whole Blood in certified metal-free EDTA tubes.

Important: we use reference ranges for adults and children. Please specify age or date of birth on sample submission sheet.

Nutritional deficiencies reduce detoxification potentials. A zinc or copper deficiency reduces SOD (Superoxide Dismutase) function, thus blocking the Phase 1 Detoxification Cycle. Chelating Agents such as EDTA or DMPS easily bind trace elements such as zinc and copper. Manganese deficiency reduces the function of the detoxification enzyme MnSOD; manganese intoxication can induce Pseudo Parkinson (which is easily treated via detoxification). Blood tests are important to adequately diagnose the nutritional status.

Micronutrient Profile (P36)
Selenium, Zinc, Copper, Iron, Calcium and Magnesium.

Extended Micronutrient Profile (P35)
Selenium, Zinc, Copper, Iron, Manganese, Magnesium and Calcium.

Toxic Profile (P49)
Aluminium, Arsenic-total, Cadmium, Lead, Mercury, Nickel, Palladium, Tin and Gadolinium.

Nutrient and Toxic Profile (P4)
Magnesium, Chromium, Cobalt, Copper, Iodine, Manganese, Molybdenum, Selenium, Vanadium, Zinc

plus Aluminium, Antimony, Arsenic, Beryllium, Bismuth, Cadmium, Lead, Mercury, Nickel, Platinum, Silver, Thallium, Uranium, Tin, Zirconium.

Download Sample Submission Sheet, fill out and send with sample. Regular mail or air mail is sufficient. Freezing is not needed, but make sure sample is adequately protected to prevent breakage.

Serum or Plasma Metals

Needed Material: 3ml Serum or Plasma

Serum or Plasma Metals (P18) 

Calcium, Magnesium, Copper, Manganese, Molybdenum, Selenium, Zinc

plus Aluminium, Antimony, Beryllium, Bismuth, Lead, Gallium, Cadmium, Nickel, Platinum, Mercury, Silver, Thallium, Tin, Uranium.

Download Sample Submission Sheet, fill out and send with sample. Regular mail or air mail is sufficient. Freezing is not needed, but make sure sample is adequately protected to prevent breakage.

Hair Mineral Analysis

Hair provides a record of past and current trace element levels.

Unlike blood, hair is an inert substance that consists of a fibrous protein and trace elements. As hair grows, nutrient and toxic elements are deposited from the blood stream into the hair follicle and hair shaft. Once a trace element has been incorporated into the hair, it remains fixed.

Sample taking is painless, and sample shipping is not time dependant.

Hair analysis testing requires meticulous sample preparation, during which the sample is freed from external contaminants. Sample analysis is performed using the same instrumentation and criteria as laboratory diagnostics require for metal testing of blood and urine.

Hair analysis, when properly performed, is a reliable measure of tissue levels. Source: Jenkins DW. Toxic Metals in Mammalian Hair and Nails. EPA Report 600, 1979.


Hair Analysis

Material needed:

To measure hair metal values reliably and with good reproducibility, the following criteria must be met:

  • Only use chemically untreated head hair.
  • If the hair has been treated, it should be allowed to grow for at least 2-3 months before new sampling.
  • Alternatives to head hair are pubic, beard or chest hair. Also see Nail Analysis
  • Do not mix different sample types (i.e. hair with nails).
  • If the hair has been treated, it should be allowed to grow for at least 2-3 months before new sampling.

Hair samples do not have to be washed before submitting to testing. We enforce strict washing procedures with metal-free solutions. We follow a multiple validation process to assure analytical results and we double-test when unusual results are noted.

View a Child’s Hair Mineral Report here

View the Extended Hair Mineral Report for adults, including rare earth elements and potentially radioactive elements here

Nail Metals

Physiologically, nail can depict the history of recent imbalances. As the American Academy of Dermatology has stated in its webpage, nail has been used as a diagnostic tool since ancient times. Nutrient deficiencies, drug reaction or poisoning can be located through nail analysis. Nail mineral analysis reflects toxic exposure as it happened during the period of growth.

Micro Trace Minerals established reference ranges for nails in the 1980s.

Nail Mineral Analysis – an alternative to hair

Fingernails and toenails are made of a tough protein called keratin. Along with hair and teeth they are an appendage of the skin. These tissues accumulate metals that circulate in the blood stream, due to the growth pattern both hair and nail reflect past or chronic exposure.

The average nail growth has been noted by different authors between 2-3mm per months, and is considered about 1/3 the growth rate of hair. A complete nail replacement may take between 3 to 6 months, and toenails require 12 to 18 months. The actual growth rate is dependent upon age, gender, season, exercise level, diet, and hereditary factors. Pregnancy causes a change in nail growth as it does in hair growth.

Heavy metals in nails – Research

Biological materials are widely used as a bio-indicator for environmental Pollution with heavy metals. Among These are hair and nails, which are also recommended by the World Health Organization (WHO) for worldwide environmental Monitoring. Heavy metals Cd, Cu, Pb and Zn were determined in fingernails and scalp hair from different sexes and ages of school children, adults and workers from polluted and unpolluted Areas of Aswan, Egypt. The results reveal that people living in polluted Areas show higher concentration of metals in nails and hair. The study proves that nails and hair can be used successfully as biological indicators for the assessment of heavy metal Pollution.



Basic profil (P9)

Needed material: 0.200gr Hand- or toenails. No nail polish, please

Sample may be placed in a standard envelope. Fill out this Submission and Request Form, enclose and send via regular air mail.After sample receipt, we need 3-4 days for testing.

(Note:list prices include 19% sales tax, applicable to European Union clients only. Those having nonexempt status, can deduct 19%)

Tested Parameter:

Boron, Calcium, Chromium, Cobalt, Copper, Germanium, Iodine, Iron, Lithium, Magnesium, Manganese, Molybdenum, Selenium, Strontium, Vanadium, Zinc

plus Aluminium, Antimony, Arsenic, Barium, Beryllium, Bismuth, Cadmium, Lead, Nickel, Palladium, Platinum, Mercury, Silver, Thallium, Tin, Titanium, Tungsten, Uranium, Zirconium.

Nail analysis – Extended Profil (P10)

This extended Profile contains all metals as found in the Basic Profile (P9) plus additional rare earth metals as utilized in industry, medicine and dentistry.

Needed Material, see Basic Profile (P9) View report here

Tested Parameter:

Boron, Calcium, Chromium, Germanium, Iodine, Iron, Cobalt, Copper, Lithium, Magnesium, Manganese, Molybdenum, Selenium, Strontium, Vanadium, Zinc

plus Aluminium, Antimony, Arsenic, Barium, Beryllium, Bismuth, Cadmium, Lead, Cesium, Cerium, Dysprosium, Erbium, Europium, Gadolinium, Gallium, Iridium, Lanthanum, Lutetium, Mercury, Nickel, Palladium, Platinum, Praseodymium, Rhenium, Rhodium, Ruthenium, Samarium, Silver, Tantalum, Tellurium, Thallium, Thorium, Thulium, Tin, Titanium, Tungsten, Uranium, Ytterbium, Zirconium.

Comparing the Metal Concentration in the Nails of Healthy and Cancer Patients Living in the Malwa Region of Punjab, India with a Random European Group – A Follow up Study

British Journal of Medicine and Medical Research, Vol. 5, Issue 4

Micro Trace Minerals Research Article Abstract:

The cancer prevalence in the Malwa region of Punjab (1089/million/year) is much higher than the national average cancer prevalence in India (800/million/year). In our previous study on hair metal analysis, we located a high metal burden in Punjabi cancer patients and their live-in relatives, suggesting that an excessive metal exposure is a factor in the pathogenesis of cancer. The present study focused on nail metal analysis, a biological material similar to hair. Previously, we had used ICP-MS spectroscopy to confirm high exposures to aluminium (Al), barium (Ba), manganese (Mn), lead (Pb), uranium (U and other metals in the hair of Punjabi cancer patients and their healthy relatives (Blaurock-Busch et al. 2014). In this study, we used nail metal analysis to confirm the results of our previous study.

We compared the nail metal concentration of healthy Punjabis (N=83) with randomly selected healthy Europeans (N=83) and found highly significant differences between the European and Punjabi groups, including the healthy and the cancer groups.In comparison, our European group showed a low percentage (0 to 13%) of pathological values for aluminium (Al), barium (Ba), cadmium (Cd), iron (Fe), manganese (Mn), nickel (Ni), lead (Pb), strontium (Sr), titanium (Ti) and uranium (U), while the healthy Punjabi groups showed between 13% and 99% pathological values for these elements.

The greatest metal burden was found in the breast cancer group (N=13), showing 100% pathological values for Al, Fe, Mn and U. This study supports previous research, which demonstrated a significant metal burden in Punjabi people. Water, soil, and phosphate fertilizers may be the cause of this excessive exposure.

Full article

Selenium in Toenails



  1. Channing Laboratory, Department of Medicine, Harvard Medical School and Brigham and Women’s Hospital Boston, MA
  2. Department of Epidemiology, Harvard School of Public Health Boston, MA
  3. Research Reactor Facility, University of Missouri Columbia, MO
  4. Department of Nutrition, Harvard School of Public Health Boston, MA

To assess the validity of the selenium concentration in human toenails as a measure of selenium intake and to determine other correlates of toenail selenium level, the authors examined toenail selenium within two subgroups of a large cohort study of US women. Mean toenail selenium was higher among 38 consumers of selenium supplements (0.904 µg/g, standard deviation (SD) 0.217) than among 96 nonusers (0.748 µg/g, SD 0.149; p<0.001), and a dose-response relation was observed among supplement users (Spearman’s r=0.32; p=0.05). In a second subgroup of 677 women, selenium supplement use was also associated with higher mean toenail selenium (0.906 µg/g, SD 0.214, among 18 users and 0.801 µg/g, SD 0.148, among 659 nonusers; p=0.02), and the dose-response relation was also significant (Spearman’s r=0.50; p=0.03). The geographic variation in toenail selenium levels was consistent with the geographic distribution of selenium in forage crops. Toenail selenium declined with age and was significantly reduced among cigarette smokers (mean=0.746, SD 0.124, among 146 current smokers and mean=0.817, SD 0.159, among 311 never smokers; p<0.001) but was not materially affected by alcohol consumption.

These data provide further evidence for the validity of toenail selenium as a measure of selenium intake and indicate the need to control for age and cigarette smoking in epidemiologic studies of the health effects of selenium exposure.

Metals in Saliva

Many studies have confirmed the corrosion of dental alloys. Garhammer et al reported that specific metals such as silver, gold, chromium and others were higher in saliva of patients with metal restorations as in control patients. The researches stated that ‘The metal content of saliva is affected among other things by intraoral metal restorations.*

Through saliva metal testing we can detect how amalgam metals of fillings or crowns are released into saliva. Systemic metal exposure should be confirmed through additional diagnostics.

* Garhammer P, Hiller KA, Reiginger T, Schmalz G. Metal content of saliva with and without metal restorations. Clinical Oral Investigations, Vol8, #4, 238-242

Amalgamfillings and Dental Materials – Cause of oral health problems?


The number of amalgam fillings and dental alloys of 86 female patients were recorded. The salivary and urinary concentrations of mercury were determinated by atomic absorption spectrometry (AAS) and the concentrations of various metallic components (silver, copper, tin, gold, palladium, platinum, cobalt, molybdenum, indium, gallium) were analysed by inductively coupled plasma mass spectrometry (ICP-MS). The saliva concentrations of mercury were also determinated after chewing gum and the urinary concentrations of mercury and tin 45 minutes after application of 250mg DMPS (dimercaptopropane sulfonate). The salivary concentrations of mercury, silver, tin and the urinary mercury excretion after DMPS correlated significantly with the number of amalgam fillings, In contrast, there was an inverse significantly correlation between the urinary tin excretion after DMPS and the number of amalgam fillings; also between the urinary tin after DMPS and mercury in saliva before chewing. Possibly there are competitive interactions between the urinary excretion of mercury and tin. Dental alloys shows no effects of the mercury release from dental amalgam. Presumable oral galvanic elements do not exist. Our results showed, that the urinary excretion of metals before DMPS is unsuitable to value the metal burden due to amalgam fillings and dental alloys.

umwelt-medizin-gesellschaft 17 (1): 57-67


Prof. Dr. med. Ingrid Gerhard, Universitäts-Frauenklinik Heidelberg, Abt. für Gynäkologische Endokrinologie und Fertilitätsstörungen, Ambulanz für Naturheilkunde, Voßstr. 9, 69115 Heidelberg; Claudia Ganzer, Palmersdorfer Hof 16, D-50321 Brühl

Saliva Testing

Saliva needed: 3ml. Ask for test kits.

Dental Profile (P3):

Cadmium, Chromium, Cobalt, Copper, Gallium, Iridium, Mercury, Molybdenum, Nickel, Palladium, Platinum, Rhodium, Silver, Tin

additional option:

Dental Profile (P3) plus Gold

Extended Dental Profile (P43):

Tested Elements Parameter as profile 3 plus:

Aluminum, Beryllium, Boron, Cerium, Iron, Lanthanum, Manganese, Niobium, Rhenium, Ruthenium, Tantalum, Titanium, Tungsten, Vanadium, Zinc, Zirconium

additional option:

Extended Dental Profile (P43) plus Gold


Sample Submission Form / Information

Saliva and Fecal Testing




Professor N. Campbell